Monday, 12 December 2011

Why Illumina reads may have uneven coverage? - SEQanswers

Check out this forum thread about cases where uneven coverage of NGS seq. (the thread is about Illumina but I think all Platforms have their own quirks)

The consensus is that GC bias and/or enrichment PCR step might be creating platform specific biases. 

Whole genome sequencing projects like in 
Complete Khoisan and Bantu genomes from southern Africa : Article : Nature where WGS data on different platforms are available have the ability to shed some light on such issues. 

So when in doubt, (and have plenty of $ ) sequence with multiple platforms. 

No comments:

Post a Comment

Datanami, Woe be me