ART: a next-generation sequencing read simulator.

http://bioinformatics.oxfordjournals.org/content/early/2011/12/23/bioinformatics.btr708.long

Bioinformatics. 2011 Dec 23. [Epub ahead of print]

ART: a next-generation sequencing read simulator.

Huang W, Li L, Myers JR, Marth GT.

Source

Biostatistics Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.

Abstract

SUMMARY:

ART is a set of simulation tools that generate synthetic next-generation sequencing reads. This functionality is essential for testing and benchmarking tools for next-generation sequencing data analysis including read alignment, de novo assembly, and genetic variation discovery. ART generates simulated sequencing reads by emulating the sequencing process with built-in, technology-specific read error models and base quality value profiles parameterized empirically in large sequencing datasets. We currently support all three major commercial next-generation sequencing platforms: Roche's 454, Illumina's Solexa, and Applied Biosystems' SOLiD.ART also allows the flexibility to use customized read error model parameters and quality profiles.

AVAILABILITY:

Both source and binary software packages are available at http://www.niehs.nih.gov/research/resources/software/art.