Thursday, 15 December 2011

Metrics for Coverage uniformity and evenness of coverage

Revisiting this issue which was mostly covered in this post using genomecoveragebed . However, chanced upon Illumina's comparison on the SBL sequencing platform version 4 with their Truseq ( I wasn't aware of an alternative name for that particular platform!)  

My question is 
Do you use similar metrics to QC between your NGS samples for possible mishandling? 

What happens when you have 100 or even 1000 of these samples? Would checking the  skewness or the kurtosis of the peaks be useful?


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