One of the top questions posted in the Galaxy User mailing list.
reposted the summary links here for convenience.
Tutorial covering RNA-seq analysis (tool under "NGS: RNA Analysis")
http://usegalaxy.org/u/jeremy/ p/galaxy-rna-seq-analysis-exer cise
FAQ to help with troubleshooting (if needed):
http://usegalaxy.org/u/jeremy/ p/transcriptome-analysis-faq
For visualization, an update that allows the use of a user-specified
fasta reference genome is coming out very soon. For now, you can view
annotation by creating a custom genome build, but the actual reference
will be not included. Use "Visualization -> New Track Browser" and
follow the instructions for "Is the build not listed here? Add a Custom
Build".
Help for using the tool is available here:
http://galaxyproject.org/ Learn/Visualization
Currently, RNA-seq analysis for SOLiD data is available only on Galaxy test server:
http://test.g2.bx.psu.edu/
Please note that there are quotas associated with the test server:
http://galaxyproject.org/wiki/ News/Galaxy%20Public%20Servers %20Usage%20Quotas
[Credit : Jennifer Jackson ]
http://usegalaxy.org
http://galaxyproject.org/Suppo rt
Another helpful resource (non-Galaxy related though) is
http://seqanswers.com/wiki/How-to/RNASeq_analysis written by Matthew Young
and the discussion on this wiki @ seqanswers
http://seqanswers.com/forums/showthread.php?t=7068
As well as this review paper in Genome Biology RNA-seq Review
Stephen mentions this tutorial as well in this blog
Dr David Matthews
has posted a starter thread to discuss RNA seq analysis workflow on
Paired End Seq with Tophat on Galaxy in the mailling list.
His post and the discussion thread is here.
http://gmod.827538.n3.nabble.com/Replicates-tt2397672.html#a2560404
kevin:waiting for the next common question to come next, is there Ion Torrent Support on Galaxy ?)
reposted the summary links here for convenience.
Tutorial covering RNA-seq analysis (tool under "NGS: RNA Analysis")
http://usegalaxy.org/u/jeremy/
FAQ to help with troubleshooting (if needed):
http://usegalaxy.org/u/jeremy/
For visualization, an update that allows the use of a user-specified
fasta reference genome is coming out very soon. For now, you can view
annotation by creating a custom genome build, but the actual reference
will be not included. Use "Visualization -> New Track Browser" and
follow the instructions for "Is the build not listed here? Add a Custom
Build".
Help for using the tool is available here:
http://galaxyproject.org/
Currently, RNA-seq analysis for SOLiD data is available only on Galaxy test server:
http://test.g2.bx.psu.edu/
Please note that there are quotas associated with the test server:
http://galaxyproject.org/wiki/
[Credit : Jennifer Jackson ]
http://usegalaxy.org
http://galaxyproject.org/Suppo
Another helpful resource (non-Galaxy related though) is
http://seqanswers.com/wiki/How-to/RNASeq_analysis written by Matthew Young
and the discussion on this wiki @ seqanswers
http://seqanswers.com/forums/showthread.php?t=7068
As well as this review paper in Genome Biology RNA-seq Review
Stephen mentions this tutorial as well in this blog
RNA seq analysis workflow on Galaxy (Bristol workflow)
His post and the discussion thread is here.
http://gmod.827538.n3.nabble.com/Replicates-tt2397672.html#a2560404
kevin:waiting for the next common question to come next, is there Ion Torrent Support on Galaxy ?)
Hi,
ReplyDeleteAny idea on how to convert BioPax file to SBML file?
If I use the converter BioPaX2SBML, I am getting following error:
mv: missing destination file operand after `/rahome/webservices/galaxy/database/files/002/dataset_2809.dat'
Try `mv --help' for more information.