http://www.ncbi.nlm.nih.gov/pubmed/21625543
Abstract Microbial cloning makes Sanger sequencing of complex DNA sample intensive. We present a simple, rapid and robust method that enable special equipment to perform single-molecule amplicon sequencing, throughput manner, from sub-picogram quantities of DNA. The meth quick quality control of next-generation sequencing libraries, as was metagenomic sample. PMID: 21625543 [PubMed -in process]
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