Friday, 12 August 2011

is 12 million 90 bp transcriptome reads enough for transcriptome assembly?

Posted a pubmed link recently, the authors "report the use of next-generation massively parallel sequencing technologies and de novo transcriptome assembly to gain a comprehensive overview of the H. brasiliensis transcriptome. The sequencing output generated more than 12 million reads with an average length of 90 nt. In total 48,768 unigenes (mean size = 436 bp, median size = 328 bp) were assembled through de novo transcriptome assembly."

Do you think such an assembly truly is useful for research? or would a higher coverage been better? 

No comments:

Post a Comment

Datanami, Woe be me