testing Tophat 1.1.2 now
on a 8 Gb Ram CentOS box managed to align 1 million reads to hg18 in 33 mins and 2 million reads in 59 mins. using 4 threads
Nice scalability! But it was slower than I was used to for bowtie. I kept killing my full set of 90 million reads thinking there's something wrong. Guess I need to be more patient and wait for 45 hours.
I do wonder if the process can be mapped to separate nodes to speed up.