http://www.ncbi.nlm.nih.gov/pubmed/22585165

Cancer Discov. 2012 Jan;2(1):23-4.

Tackling formalin-fixed, paraffin-embedded tumor tissue with next-generation sequencing.

Corless CL, Spellman PT.

Source

Departments of Pathology and Molecular and Medical Genetics, and Knight Cancer Institute, Oregon Health & Science University, Portland, Oregon.

Abstract

Most tumor samples available for clinical genotyping are formalin-fixed and paraffin-embedded (FFPE), but there has been relatively little published on the suitability of such samples for next-generation sequencing approaches. A new study by Wagle and colleagues shows that a combination of hybridization-capture and deep sequencing yields high-quality data from FFPE specimens. Cancer Discovery; 2(1); 23-4. ©2012 AACR.

PMID:

 

22585165

 

[PubMed - in process]