Sequencing Difficult Templates - Why Quality is Everything
Date:
Wednesday, November 6
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Time: 1:00 pm A.E.D.T
Speaker:
Josquin Tibbits, PhD,
Senior Research Scientist, Dept of Environment and Primary Industries
Abstract
For most applications, sequence quality (low error rates, correct library size, even coverage etc), stands out as the key metric for the downstream utility of data from NGS platforms. I investigate the quality and utility of data generated from a range of platforms (454, HiSeq, MiSeq and PGM) for the reference initiated assembly of homopolymer, repeat and low complexity plant plastid genomes. These types of sequences are a good proxy for the more difficult sequence regions found when exploring larger genomes in both agricultural and human sequencing projects. The analysis will show in detail how the different platforms cope with these challenging regions
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