Biome | Q&A with Rich Roberts on single-molecule sequencing technology

The exciting part about single molecule sequencing for me was the ability to sequence low abundance transcripts or have phased haplotypes for human sequencing. Having a high error rate nullifies any advantage in these areas. But I guess it's a tool in the end and how you use it to get meaning results.


"As the previously rapid climb in cost efficiency brought about by next-generation sequencing plateaus, the failure of single-molecule sequencing to deliver might leave some genomics aficionados despondent about the prospects for their field. But a recentCorrespondence article in Genome Biology saw Nobel laureate Richard Roberts, together with Cold Spring Harbor’s Mike Schatz and Mauricio Carneiro of the Broad Institute, argue that the latest iteration of Pacific Biosciences’ SMRT platform is a powerful tool, whose value should be reassessed by a skeptical community.

In this Q&A, Roberts tells us why he thinks there’s a need for re-evaluation, and what sparked his interest in genomics in the first place."

http://www.biomedcentral.com/biome/rich-roberts-discusses-single-molecule-sequencing-technology/

Correspondence

The advantages of SMRT sequencing

Roberts RJ, Carneiro MO and Schatz MC

Genome Biology 2013, 14:405

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