Abstract
Recent exponential growth in the
throughput of next-generation DNA sequencing platforms has dramatically
spurred the use of
accessible and scalable targeted resequencing
approaches. This includes candidate region diagnostic resequencing and
novel
variant validation from whole genome or exome
sequencing analysis. We have previously demonstrated that selective
genomic
circularization is a robust in-solution approach
for capturing and resequencing thousands of target human genome loci
such
as exons and regulatory sequences. To facilitate
the design and production of customized capture assays for any given
region
in the human genome, we developed the Human
OligoGenome Resource (
http://oligogenome.stanford.edu/).
This online database contains over 21 million capture oligonucleotide
sequences. It enables one to create customized and
highly multiplexed resequencing assays of target
regions across the human genome and is not restricted to coding regions.
In total, this resource provides 92.1%
in silico
coverage of the human genome. The online server allows researchers to
download a complete repository of oligonucleotide probes
and design customized capture assays to target
multiple regions throughout the human genome. The website has query
tools for
selecting and evaluating capture oligonucleotides
from specified genomic regions
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